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KMID : 0525720220270020085
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Journal of Chitin and Chitosan
2022 Volume.27 No. 2 p.85 ~ p.93
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Acetylated Distarch Adipate Ameliorates Gastrointestinal Permeability Via Reduction of Inflammation in Human Colon Epithelial Cells
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Kim Min-Gyeong
Cho Chi-Heung
Kim Se-Ra
Oh Mi-Jin
Park Ho-Young
Lee Sang-Hoon
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Abstract
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The intestine digests the food we eat and regulates the absorption of nutrients as well protect the body from external antigen or toxic substances. This barrier function become abnormal during inflammation. In this study, we confirmed the anti-inflammatory and epithelial barrier protective properties of acetylated distarch adipate (ADSP) in human-derived intestinal epithelial cells. ADSP showed a significant increase in cell viability with pro-inflammatory cytokine treatment and non-treatment. IL-6 and IL-1β significantly decreased with ADSP treatment as compared to the inflammation-induced group, while IL-8 didn’t show significant change. After confirming that ADSP significantly reduces the epithelial monolayer integrity damage and increased permeability induced by TNF-α and IFN-γ, we confirmed the change in expression amount of tight junction with immunofluorescence and western blotting. Our results contributed to the study of the bioactivity of ADSP, which is widely used as a food additive, and indicated its potential as an agent for inflammatory bowel disease.
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KEYWORD
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Acetylated distarch adipate, Modified starch, Inflammatory bowel disease, Tight junction, Caco-2
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